Table 1.

Oligonucleotide Primer and Probe Sequences Used in the GeneXpert Assay

Gene	Oligonucleotide	Sequence
GUSB	RT primer	5′-TTTGGT TGTCTCTGCCGAGT-3′
	Forward primer	5′-CTCATTTGGAATTTTGCCGATTTCA-3′
	Reverse primer	5′-CCGAGTGAAGATCCCCTT-3′
	Probe	5′-TGACTGAACAGTCACCGACGAGAGTGCTGG-3′
PIP	RT primer	5′-GGGAATGTCAAAATTCTTT-3′
	Forward primer	5′-GCGAGCTCCAGCTCCTGTTCAG-3′
	Reverse primer	5′-GGCGCAATTATGATCTTCCGAGTGTTGTC-3′
	Probe	5′-CCAGCCCTGCCACCCTGCTCCTG-3′
TACSTD1	RT primer	5′-AGTTTACGGCCAGCTTG-3′
	Forward primer	5′-GACGCGTTCGGGCTTCTGCTT-3′
	Reverse primer	5′-GGCGAAGTTTTCACAGACACATTCTTCCTGAG-3′
	Probe	5′-CGGCGACGGCGACTTTTGC-3′

Bases indicated in bold are noncomplementary tails added to increase primer melting temperature in later PCR cycles.