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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Neuroscience. 2009 Jul 24;163(3):877–889. doi: 10.1016/j.neuroscience.2009.07.012

Figure 2. Effects of methylene blue on rotenone-induced striatal damage in unilaterally infused rats.

Figure 2

(A and B) Intrastriatal infusion of rotenone alone (Rot) produced a large area of liquefactive necrosis surrounded by gliosis (asterisk) and accompanied by mild ipsilateral lateral ventricle enlargement (arrow). The toxic effects of Rot appeared to be attenuated in those rats receiving a combined intrastriatal infusion of Rot/MB. (C and D) Peri-lesional oxidative stress in situ was measured in micrographs of DHE-stained sections. An increased signal was detected in the striatum adjacent to the lesion in the Rot-treated subjects. Conversely, the DHE signal was comparatively lower in penumbral regions in Rot/MB-treated subjects. Epifluorescence microscopy, 50X. (E and F) Cytochrome oxidase histochemistry-stained forebrain coronal sections from the same subjects depicted in A and B show corresponding regions of striatal hypometabolism that is less severe in the Rot/MB-treated subject.