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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Circ Res. 2009 Aug 6;105(5):481–491. doi: 10.1161/CIRCRESAHA.109.203703

Figure 1. Nampt is downregulated by pathological stimuli in vivo.

Figure 1

Figure 1

(A) Heart homogenates were prepared from mice subjected to ischemia for 24 hours, ischemia for 45 minutes and reperfusion for 24 hours, and transverse aortic constriction (TAC) for 2 or 4 weeks. Expression of Nampt, GAPDH and actin was evaluated by immunoblots. (B) The effect of pathological stress on the expression level of Nampt mRNA in the heart. Nampt mRNA level was determined by RT-qPCR and normalized by S15RNA. The expression level of sham operated mice is expressed as 1. (C) Representative Nampt stainings in heart sections from sham operation and ischemia/reperfusion are shown. (D) Heart homogenates were prepared from mice with indicated ages. Expression of Nampt and tubulin was evaluated by immunoblots.