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. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Circ Res. 2009 Aug 6;105(5):481–491. doi: 10.1161/CIRCRESAHA.109.203703

Figure 5. Generation of Tg-Nampt mice.

Figure 5

(A) Representative immunoblots of heart homogenates obtained from Tg-Nampt and NTg mice with anti-Nampt and anti-tubulin antibodies. (B) Representative immunostaining of Nampt in left ventricular myocardial sections. (C) The level of [NAD+], [NADH] and [NAD+]/[NADH] in NTg and Tg-Nampt (line #9) was evaluated. Data represent the mean of 5–6 experiments ± SEM. (D) Tg-Nampt (line 8) or NTg control mice were subjected to ischemia or sham operation for 24 hours. Expression of Nampt and tubulin was determined by immunoblot analyses. The results shown are representative of three experiments.