Table 2.
TCE metabolitesa reported at 2 hr after exposure to TCE.
| Dose (mg/kg, route) |
DCA (nmol/ml) |
TCA (nmol/ml) |
Matrix | Species | Strain | Assay method | LOD on column (pmole) |
Reference |
|---|---|---|---|---|---|---|---|---|
| TCE (2140, oral*) | 0.122 | 256 | Plasma | Mouse | B6C3F1 | LC-MS/MS | 0.075 (DCA LOD) | Present study |
| 3.00 (TCA LOD) | ||||||||
| 0.0375 (DCVG LOD) | ||||||||
| 0.0375 (DCVC LOD) | ||||||||
| TCE (2000, oral#) | 0.306 | NR | Blood | Rat | SD | LC-MS/MS | 0.72 (DCA LOD) | (Delinsky et al., 2005b) |
| TCE (1000, oral#) | ND | NR | (Merdink et al., 1998) | |||||
| TCE (100, iv) | NR | 110 | Blood | Mouse | B6C3F1 | GC-MS | 2.00 (DCA LOD) | |
| CH (50, iv) | NR | 200 | 0.04 (TCA LOD) | |||||
| TCE (5.8, inh)b | ND | 18 | Plasma | Human | NR | MS/MS | 0.30 (DCA LOD) | (Brashear et al., 1997) |
| TCE (1970, oral*) | NDc | NDc | Blood | Rat | F344 (m) | GC-ECD (TCA, DCA), LC-UV (DCVG, DCVC) | (Lash et al., 2006) | |
| 0.05 (DCA LOD) | ||||||||
| 0.05 (TCA LOD) | ||||||||
| 5.0 (DCVC LOD) | ||||||||
| F344 (f) | 50 (DCVG LOD) | |||||||
| TCE (2000, oral*) | NRd | 260 | (Abbas and Fisher, 1997) | |||||
| TCE (1200, oral*) | 1.7 | 355 | 0.40 (DCA LOD)e | |||||
| TCE (600, oral*) | 1.2 | 171 | Blood | Mouse | B6C3F1 | GC-ECD | 0.30 (TCA LOD)e | |
| TCE (300, oral*) | 1.4 | 263 | ||||||
| TCE (1970, oral#) | 14 | 410 | Blood | Mouse | B6C3F1 | (Larson and Bull, 1992) | ||
| TCE (3020, oral#) | ND | 62 | Blood | Rat | SD | GC-ECD | NRf | |
ND, not detected; NR, not reported; LC-MS/MS, liquid chromatography-tandem mass spectrometry; GC-MS, gas chromatography-mass spectrometry; GC-ECD, gas chromatography-electron capture detector; LC-UV, liquid chromatography-ultraviolet detector; LOD, limit of detection; LOQ, limit of quantitation.
DCVG (0.037 nmol/ml) and DCVC (0.0024 nmol/ml) are not shown here due to the sparseness of the measurements while Lash et al. (2006) reported DCVC of 0.009 nmol/ml in blood of rats after TCE treatment by oral gavage at 1970 mg/kg.
Dose in ref. (Lin et al., 2001) was estimated as follows: alveolar ventilation 2.7 L/hr/kg × concentration 0.537 mg/L (100 ppm) × exposure period 4 hr.
This study used three doses: 260 mg/kg, 660 mg/kg and 1970 mg/kg on female (f) and male (m) F344 rats, and assessed multiple time points. Although TCA in blood was below the LOD at any time point for 1970 mg/kg dosage, it was 0.124 nmol/ml (at 24 hr, female) and 9.67 nmol/ml (at 48 hr, female) for 660 mg/kg dosage; 15.8-18.2 nmol/ml (at 2-8 hr, female). The inconsistent dose-response relationship was also observed for DCVG in blood. Rats produced more DCVG between 2 and 48 hr for 263 mg/kg dosage than for 657 mg/kg; however, DCVG was detected about 0.01 to 0.08 nmol/ml between 4 and 48 hr for higher dosages, where females produce higher amounts.
Not reported at 2 hr (next available observation, 15 nmol/ml at 4 hr).
LOD on column imputed by an original method(Ketcha et al., 1996) due to lack of information.
LOD on column not reported. Limit of detection on sample-base were 4 nmol/ml of DCA and 15 nmol/ml of TCA.
Administered in corn oil as vehicle
Administered in an aqueous emulsion as vehicle.