Table 1.

Summary of in situ hybridization results for 44 genes characterized for effects of age, song exposure, and sex. Genes are indicated with their individual ESTIMA database gene identifiers, and are organized based on what microarray statistical group they were selected from. Check marks indicate genes for which there was a significant main effect (p < 0.05) of age, song, or sex by in situ hybridization; dashes are those genes for which there was no significant main effect. Check mark plus asterisk indicates the one gene that in post-hoc test showed a difference between P20 and P45 expression levels. Check mark plus cross indicates the one gene that showed a significant song response in P45 birds.

ESTIMA identifier	age	song	sex
development
SB03049A2G10.f1	√	–	–
SB03011B2G05.f1	√	–	–
SB010003000C11	–	–	√
SB02031B1E08.f1	√	–	–
SB02028B1B03.f1	–	–	–
SB02003A1D09.f2	√*	–	√
SB03013A1G06.f1	√	–	–
SB03014A1B02.f1	√	–	–
SB02013B2G03.f1	√	–	–
SB02018B1D11.f1	√	–	–
SB03047A1F11.f1	√	–	–
adult song vs. adult silent
SB02016B1B10.f1	√	√	–
SB02035B2E10.f1	√	–	–
SB03001A1D09.f1	√	√	√
SB02018A1F12.f1	√	√	–
SB03019A1F04.f1	√	√	–
SB03001B2H03.f1	√	–	–
SB03033A2C05.f1	–	√	–
SB03026B1F05.f1	√	–	–
SB02019A1D12.f1	√	√†	–
P20 song vs. P20 silent
SB03042B2D08.f1	√	–	–
SB03028A1A09.f1	–	–	√
SB02039A2E12.f1	√	–	–
SB02045A2F12.f1	√	–	–
SB03013B1E10.f1	√	–	–
SB03038B2A04.f1	√	√	√
SB03033A1D10.f1	√	–	–
SB03026B1B08.f1	–	–	–
SB03002A1A11.f1	√	–	–
SB03009B1A05.f1	–	–	–
SB02019B2E04.f1	√	–	–
interaction
SB02033B1G04.f1	–	–	–
SB02025B1A09.f1	√	–	–
SB03039B1B11.f1	–	–	–
SB010006000A04	√	√	√
SB03047B2G09.f1	√	–	–
SB02034A1B02.f1	–	–	–
SB02021A1C06.f1	–	–	–
SB02016B1B10.f1	√	–	–
SB02020A2A08.f1	√	–	–
SB02027B1A05.f1	√	–	–
SB02012A1E04.f1	–	–	√
SB02028A1C05.f1	√	–	√
SB03022B1B08.f1	–	–	–