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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Immunity. 2009 Jul 17;31(1):47–59. doi: 10.1016/j.immuni.2009.04.018

Figure 6.

Figure 6

Binding of mutant NKT TCRs as assessed by CD1d-α-GalCer tetramer inhibition. Recombinant soluble NKT TCRs, and mutants thereof, were examined for their ability to block binding of mCD1d-α-GalCer tetramers to mouse NKT cells. PE labelled CD1d-α-GalCer tetramers were pre-incubated with titrating amounts of soluble wild type and mutant NKT TCRs before staining of mouse thymocytes. Cells were analysed by flow cytometry showing mCD1d-α–GalCer tetramer-PE on the vertical axis and anti-CD3 APC on the horizontal axis. CD3+ mCD1d-α–GalCer tetramer+ thymic NKT cells are indicated within the square with the MFI indicated.