Tabel 1.
Oxidizing agent | MX 0mM | MX 1mM | Mx 2mM | MX 5mM | MX 10mM |
---|---|---|---|---|---|
H2O2 (uM) LD50 | 220 ± 7 | 125 ± 2* | 112 ± 2*# | 85 ± 4*# | |
§menadiones (uM) LD80 | 44 ± 3 | 36 ± 2* | 36 ± 2* | 36 ± 1* | |
XO (mU/ml)/HX LD50 | 17 ± 3 | 4 ± 0.2* | 2.4 ± 0.1*# | 1.6 ± 0.2*# |
Note: The values are LD50 of H2O2 and XO/HX and LD80 of menadione or without MX treatment for 24hrs (n=3).
Abbreviations: H2O2, hydrogen peroxide; XO, Xanthine Oxidase; HX, hypoxanthine; MX, methoxyamine.
P< 0.05, all compared each +/− MX to oxidant alone at LD50 dose.
P< 0.05, all compared each + higher dose MX to oxidant + lower MX dose at LD50 dose.
LD80menadione