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. Author manuscript; available in PMC: 2009 Oct 22.
Published in final edited form as: Anal Chem. 2009 Sep 1;81(17):7149–7159. doi: 10.1021/ac900360b

Scheme 2. Characterization of Cross-Linkers a.

Scheme 2

a (a) Thiol recovery in a SAMS-crosslinked peptide. The thiol is protected with an acetyl group in crosslinked peptides (IX) that is removed by N-hydroxylamine to form thiol recovered cross-linked peptides (X). (b) UV cleavage of the photo cleavable crosslinker after modification. Crosslinked peptides were anchored on magnetic beads using a photocleavable spacer (XI). After UV cleavage, the enriched peptides (XII) were released and the photocleavable cross-linked formed nitrosobenzyldehyde (XIII).