Fig. 3.
LFA-1 nanoclusters are spatially segregated but proximal to GPI-AP hotspots. (A) Representative dual color excitation/detection NSOM image of LFA-1 nanoclusters (red) and GPI-APs (green) in steady state. (B) Nearest inter-domain distance distributions of LFA-1 nanoclusters to its closest GPI-AP (bars) together with simulations of random spatial distribution of LFA-1 nanoclusters and GPI-APs (red). The inset corresponds to the difference (Δi in %) between experimental data and simulations. At shorter distances (< cross-over point in Δi) both distributions are significantly different with P < 0.01. Nearest inter-domain distance distributions of: (C) GPI-AP monomers to its closest LFA-1 nanocluster (bars); and (D) GPI-AP nanodomains to its closest LFA-1 nanocluster (bars), together with the corresponding simulations (red). Experimental and simulation distributions in (C) are significantly similar with P = 0.22 while in (D) are significantly different, P < 0.008. Cartoons and arrows direction next to the distributions indicate how the interdomain distances were measured.