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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2009 Jul 10;29(11):1779–1786. doi: 10.1161/ATVBAHA.109.191759

Figure 4. Macrophage Chemotactic Activity in Metabolically Stress Mice.

Figure 4

(A) Matrigel plugs filled with either vehicle (left bar, number of cells given in parentheses) or MCP-1 (300 nM, right bar) were injected into the left and right flanks, respectively, of LDL-R−/− mice exposed to either mild (MD, □ n=5), moderate (HFD, ■, n=5) or severe metabolic stress (STZ+HFD, ■, n=10). After three days the plugs were removed, dissolved and cells were counted. Immunostaining with antibodies directed against macrosialin/CD68 confirmed that > 93% of the cells recruited into the Matrigel plugs were macrophages. (B) Correlation between glutathione reduction potential (Eh) of peritoneal macrophages isolated from mildly (MD, Ο, n=5), modestly (HFD, ♦, n=5) and severely metabolically-stressed LDL-R−/− mice (STZ+HFD, ▴, n=10) and macrophage numbers recruited into Matrigel plugs implanted for 3 days into the same animals (r2=0.554, P<0.001).