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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Br J Dermatol. 2009 Apr 29;161(2):237–248. doi: 10.1111/j.1365-2133.2009.09215.x

Fig 2.

Fig 2

Transforming growth factor (TGF)-β1 protein expression in interleukin (IL)-6 knockout (KO) fibroblast-populated lattices. Floating collagen matrices were prepared using primary dermal fibroblasts from IL-6KO mice as described. The growth medium was replaced and cultures were treated with various concentrations of recombinant mouse (rm) IL-6 overnight. Total protein was prepared, and protein expression was determined with an enzyme-linked immunosorbent assay as per the manufacturer’s instructions (eBioscience). Data are expressed as mean ± SD (n = 9 lattices). *Significantly different from 0 ng mL−1 rmIL-6, P ≤ 0·05; white bar, lattice; black bar, supernatant.