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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Br J Dermatol. 2009 Apr 29;161(2):237–248. doi: 10.1111/j.1365-2133.2009.09215.x

Fig 4.

Fig 4

Immunohistology of transforming growth factor (TGF)-β1 and TGF-β type 2 receptor (TGF-βR2) in intradermal injection skin samples. Interleukin (IL)-6 knockout (KO) mice were injected with 10 μL of 0 ng mL−1 (a, c) or 100 ng mL−1 (b, d) recombinant mouse (rm) IL-6, and mRNA was extracted from the tissue as described. Formalin-fixed, paraffin-embedded tissue sections of treated skin samples were incubated with anti-TGF-β1 (a and b), anti-TGF-βR2 (c and d) antibodies, and detected using Alexflor 546 conjugated secondary antibodies. Stained slides were visualized under fluorescence using a Leica DM400B microscope and 20 × objective.