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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Br J Dermatol. 2009 Apr 29;161(2):237–248. doi: 10.1111/j.1365-2133.2009.09215.x

Fig 6.

Fig 6

Inhibition of the Erk1/2 signal transduction pathway affects transforming growth factor (TGF)-β1 expression. Floating collagen matrices were prepared using primary dermal fibroblasts from interleukin (IL)-6 knockout (KO) mice as described. The growth medium was replaced and cultures were treated with various concentration of LLL-3 (Stat3 inhibitor), PD98059 (Erk inhibitor) or vehicle [0·1% dimethyl sulphoxide (DMSO) in culture media] 30 min prior to recombinant mouse (rm) IL-6 treatment as described. Expression of mRNA was determined by real-time polymerase chain reaction utilizing an ABI 7000 SDS system and accompanying software. Expression differences were determined relative to 28S rRNA utilizing the ΔΔCt method. Data are expressed as mean ± SE (n = 6 lattices per data point). White bar, DMSO; grey bar, PD98059; black bar, LLL-3.