Figure 6.

Anti-Porimin defines a 110-kDa antigen in the lysate of Jurkat cells by immunoblot (A) or immunoprecipitation assay (B). For immunoblotting assay, cell lysates from Jurkat subclones E6–1, JA1, J. Cam1.6, and J45.01 (lanes 1–4) or from H9 (lane 5), CEM (lane 6), or K562 cells (lane 7) were blotted with anti-Porimin and visualized by chemiluminescence assay. The relative migration of protein standards is indicated on the right. For immunoprecipitation assay, Jurkat cells were biotinylated and then lysed in a lysis buffer. Immunoprecipitates from the cell lysates by anti-Porimin (lane 1) or control antibody MsIgM (lane 2) were analyzed by Western blotting assay by using streptavidin-peroxidase conjugate.