Bundle sheath development from clearings of C3 and C4 Flaveria leaves. (A, C, E) Bundle sheath cells are indistinct from mesophyll in developing leaves of C3 F. robusta. Insets with arrows show enlargement of bundle sheath cells and lack of distinguishing morphological features. (B, D, F) Bundle sheath cells are distinct from mesophyll in developing leaves of C4 F. bidentis. Insets with arrows demonstrate bundle sheath cells enlarging in perpendicular diameter in younger leaves, and in parallel diameter in older leaves. Scale bar: all images = 100 µm; insets are 2×.