Fig. 1.

Site of T-DNA insertion in Atsuc2-4 (SALK_038124) and expression cassettes used for, and results of, [¹⁴C]Suc uptake into yeast. (A) T-DNA insertion site in the second intron of AtSUC2 (At1g22710) in Atsuc2-4 with flanking nucleotides indicated; exons are indicated as white boxes and intergenic regions are indicated by the solid black line; numbering is relative to the start of the 5′ UTR, based on the gene model AT1G22710·1 at http://www.arabidopsis.org; T-DNA is indicated as labelled above the gene model. (B) The three expression cassettes used for [¹⁴C]Suc uptake into yeast cells; full-length and truncated AtSUC2 cDNA as labelled with fused exons indicated as white boxes; ATG start and TGA stop codons as labelled; MCS, multiple-cloning site in the empty-vector control, pRS424::ADH-MCS; ADHp, yeast ALCOHOL DEHYDROGENASE promoter; ADHt yeast ADH terminator. (C) Uptake of [¹⁴C]Suc uptake into yeast cultures harbouring the indicated plasmids.