Table 2.

Atsuc2-4 plants produce viable seeds and seedlings to complete their life cycle

Seed line*	Siliques with seeds	Length of siliques with seeds (mm)†	Seeds per plant‡	Seed size (mm2)§	Germination (%)
KO1	11	9·2 ± 2·1	211	0·086 ± 0·022	89
KO2	13	10·3 ± 1·8	207	0·082 ± 0·019	92
KO3	8	9·4 ± 2·3	192	0·078 ± 0·026	41
KO4	11	8·8 ± 1·8	160	0·086 ± 0·023	29
KO5	10	8·7 ± 2·0	153	0·088 ± 0·025	79
KO6	10	9·3 ± 1·8	146	0·088 ± 0·022	6
KO7	9	10·5 ± 1·4	124	0·060 ± 0·024	98
KO8	4	9·0 ± 2·7	77	0·102 ± 0·027	86
KO9	2	6·5 ± 0·7	22	0·090 ± 0·013	100
KO average	9·8 ± 3·7	9·4 ± 2·0	143 ± 62	0·084 ± 0·011	69 ± 34
WT1	172	11·8 ± 1·4	7500	0·085 ± 0·016	99
WT2	206	12·2 ± 1·2	9800	0·089 ± 0·011	97
WT3	157	12·3 ± 1·4	9500	0·091 ± 0·010	96
WT4	336	11·9 ± 1·4	8500	0·092 ± 0·010	99
WT5	321	14·7 ± 1·1	7700	0·093 ± 0·010	98
WT6	152	12·3 ± 1·4	10750	0·110 ± 0·010	100
WT average	224 ± 83	12·3 ± 1·7	8950 ± 1300	0·092 ± 0·013	98 ± 1

*An additional three Atsuc2-4 plants (KO10–KO12) flowered but did not produce seeds and are not included in this analysis. Note that plants used to generate this data are not the same as those in Table 1.

^†For calculating variation in silique length, n = number of siliques with seeds for each Atsuc2-4 plant and n = 20 for each wild-type plant.

^‡Seeds from Atsuc2-4 plants were counted individually; seeds from wild-type plants were calculated by mass assuming 50 seeds per milligram (Weigel and Glazebrook, 2002).

^§For calculating variation in seed size, n = total number of seeds from each Atsuc2-4 plant and n > 50 seeds from each wild-type plant.