Fig. 2.

FTY720 analogs promote TER barrier enhancement. A, HPAEC plated on gold electrodes were stimulated with 1 μM S1P (black line), FTY720 (red), 1R (blue), 2R (green), or 3R (purple) at time = 0. The TER tracing represents pooled data (±S.E.M.) from four independent experiments. Bar graphs depict pooled TER data from HPAEC stimulated at 1 (B) or 10 μM (C) with S1P, FTY720, 1R, 1S, 2R, 2S, 3R, or 3S as indicated. The data are expressed as maximal percentage TER change (±S.E.M.) obtained within 60 min. Positive values indicate barrier enhancement. Negative values indicate barrier disruption. n = 3–5 independent experiments per condition; ∗, p < 0.01 versus other conditions.