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FTY720 analogs do not stimulate intracellular calcium release. Cultured HPAEC were stimulated with methanol vehicle or 1 μM S1P, FTY720, 1R, 2R, or 3R at time 0, and intracellular calcium levels were measured as fold change in [Ca2+] relative to 60-s average before treatment, as determined by Fura-2 as described under Materials and Methods. n = 3 independent experiments per condition..
