Fig. 7.

Vandetanib enhances SAHA-induced cell proliferation inhibition. A, logarithmically growing glioma cells were incubated with or without varying concentrations of SAHA with or without 2 μM vandetanib for 3 days. The relationship between SAHA concentration and cell numbers was assessed semiquantitatively by spectrophotometric measurement of MTS bioreduction in T98G, U373, A172, and LNZ428, established malignant human glioma cell lines. Points represent the mean of three measurements ± S.D. There was a dose-dependent reduction in cell growth and addition of 2 μM vandetanib potentiated the SAHA-induced toxicity. No significant inhibition was detected in control cells treated with equivalent concentrations of vehicle (DMSO) or 2 μM vandetanib alone (see Fig. 2A for vandetanib dose response).