Figure 6.
Effect of IGF-1R inhibition on drug sensitivity in drug resistant cells. Multidrug resistant cell line U-2OSMR was transfected with On-Targetplus SMARTpool IGF-1R siRNA as well as non-specific siRNA. A: Confirmation of IGF-1R protein knockdown by Western blot. Total protein was isolated 72 hours post-transfection and IGF-1R, pIGF-1R and Pgp1 expression was analyzed by Western blotting. B: The relative sensitivity of each treatment to doxorubicin was determined by MTT analysis 96 hours post-transfection. C: Effects of IGF-1R knockdown by siRNA on PPP sensitivity inU-2OSMR cells.