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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Biochem J. 2008 Nov 1;415(3):367–375. doi: 10.1042/BJ20080779

Figure 2. Subsite preferences of DUBs – IsoT, UCH-L3, OTU-1 and PLpro.

Figure 2

The enzyme concentration was in the range 1–5 µM. ACC production was monitored using an fmax multi-well fluorescence plate reader (Molecular Devices) at excitation wavelength of 355 nm and an emission wavelength of 460 nm. Assay time 15–30 min. The x-axis represents the one letter code of natural amino acids (O - nor-leucine, bA-beta-alanine). The y-axis represents the average relative activity expressed as a percent of the best amino acid.