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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: J Neurochem. 2007 Aug;102(4):1329–1345. doi: 10.1111/j.1471-4159.2007.04635.x

Fig. 3.

Fig. 3

Some GABAAR in synaptosomal plasma membrane are associated with DRMs. The SPM fraction was extracted by 1% Triton X-100 at 4°C and the soluble (TS) and insoluble (TI) fractions were collected. The low-density DRM fraction was also collected after flotation in a discontinuous sucrose gradient. A: Immunoblots with rabbit antibodies to α1, α2, β2, β3, γ2 GABAAR subunits, gephyrin, transferrin receptor (TrsfR) and lipid raft markers Thy-1, Caveolin-1 and Flotillin-1. Same amount of total protein (4μg/lane) was used for SDS-PAGE. B: [3H]MUS and [3H]FNZ binding to GABAARs in TS, TI and DRM. Specific activity values (pmol/mg protein) are mean ± S.E.M of at least three experiments each done in triplicate. *** p < 0.001; ** p < 0.01. The p values are for the TS or TI fractions compared to DRM.