Figure 3.

Schematic representation of mKate and cpmKate variants. (A) Ribbon representation of the crystal structure of mKate (Pletnev et al., 2008). Note the color code for each beta sheet and alpha helix. The circular permutation sites at amino acid residues 144 and 180 are highlighted as a stick representation. (B) Schematic primary structure of mKate based on (A). (C) Design of cpmKate variants circularly permuted at amino acid residues corresponding to position 144 (upper line) and 180 (lower line). The linkers are shown in between the original N- and C-termini.