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ON and OFF time response properties of cpmKate(180)-based voltage sensors. The ΔF/F values represent the total change in fluorescence acquired from voltage-clamped PC12 cells upon test potentials of −140 to 60 mV at 35°C.
| τON at 20 mV (ms) | V1/2 (τON) (mV) | ΔF/F (τON) (%) | τOFF at 20 mV (ms) | V1/2 (τOFF) (mV) | ΔF/F (τOFF) (%) | |
|---|---|---|---|---|---|---|
| VSFP(C)cpmKate(180) | 57.1 ± 3.1 | −83.1 ± 3.4 | 1.9 | 125.0 ± 8.0 | −90.8 ± 17.2 | 1.7 |
| VSFP(D)cpmKate(180) | 64.0 ± 3.4 | −87.2 ± 2.7 | 1.2 | 174.4 ± 4.3 | −89.0 ± 5.2 | 1.3 |
