Figure 6.

Immunofluorescent images from the 22-year-old monkey illustrating colocalization of polysialylated neural cell adhesion molecule (PSA-NCAM) and neuron-specific nuclear protein (NeuN) in DCX+ cells (including bisbenzimide nuclear stain in D and H) in the prefrontal (MOG, A–C) and temporal lobe cortex (D–K). PSA-NCAM and DCX are colocalized in individual cells as well as cells arranged in chains perpendicular (A–C) or tangential (D) to the cortical surface. NeuN is detectable in a small number of DCX+ cells with relatively larger size and lighter DCX reactivity (arrows in I–J, and enlarged circles in G) than those lack colocalization (arrowheads in I–K). Note DCX+ tracts and migratory chains in layers I (E–G) and II (H). Abbreviations are the same as in Figure 2. Scale bar = 100 μm in (A–C), equivalent to 200 μm for (E–G), 50 μm for (D,I–K).