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Immunoblot of retinal homogenates of wild-type, and Rpgrip1nmf247mice (P16), with antibodies against the N-terminal coiled-coil (Ab#22) and C-terminal RID (Ab#38) domains of RPGRIP1. The absence of the ∼200 kDa full length (arrow, A) and smaller ∼150kD (arrowhead, A) and other smaller (70 and 25 kDa, B) RPGRIP1 isoforms is observed in Rpgrip1nmf247 mice. HSC70 serves as a loading control in both panels.
