Figure 3.

Effect of uPAR 3′ UTR on degradation of a stable mRNA. (A) Jurkat cells were cotransfected with the pEF-BOS-CAT normalization construct and either pBBB or pBBB 3′ uPAR. Cells were fetal bovine serum-stimulated after a 24 h serum starvation, after which poly(A)+ RNA was isolated at the indicated time points for sequential β-globin and CAT Northern analyses. The larger β-globin mRNA size in the pBBB 3′ uPAR samples reflects the additional 263 nt of 3′ uPAR cloned into the pBBB construct. Results are representative of six separate experiments. (B) β-globin Northern signals from A were densitometrically analyzed and normalized to CAT signals. Data represent percentage of maximal (90 min) counts.