TABLE 1.

Illegitimate (α × α) mating of strains with low levels of α-DNA polymerase

Experimental genotypea	Test mater genotypea	Frequency of illegitimate mating (×10−5)b	Frequency of class 1 (×10−5)c	Frequency of class 2 (×10−5)c	Frequency of class 3 (×10−5)c
GAL-POL1	Wild type	360 (270–680)d	14	184	162
GAL-POL1	mre11Δ	148 (132–165)	73e	31	44
GAL-POL1 mre11Δ	mre11Δ	156 (138–174)	64f	39	53
GAL-POL1	mre11-H125N	223 (178–268)	13	95	115
GAL-POL1 mre11-H125N	mre11-H125N	140 (115–165)	6	60	74
GAL-POL1	sae2Δ	164 (137–191)	10	87	67
GAL-POL1 sae2Δ	sae2Δ	79 (59–99)	8	17	54
GAL-POL1	rad52Δ	170 (132–208)	29	39	102
GAL-POL1 rad52Δ	rad52Δ	40 (35–46)	14	25.5	0.5

^aAll experimental strains were isogenic with MS71, a LEU2 derivative of AMY125 (MATα ade5-1 leu2-3 trp1-289 ura3-52 his7-2) (Kokoska et al. 2000), except for the GAL-POL1 gene and the indicated mutation. The mating-type tester strains are isogenic with 1225 (MATα his4-15 leu2 thr4 ura3-52 trp1 lys) except for the indicated mutation.

^bThe frequency of illegitimate mating is corrected for viability and normalized to the level of legitimate mating. Numbers in parentheses indicate the 95% confidence interval from 8–10 different cultures.

^cFifty to 100 independent illegitimate diploids were examined for each mating to determine the relative frequencies of classes 1, 2, and 3.

^dValues reported by Lemoine et al. (2005).

^eIncludes class 1B events (defined in Figure 2), which occurred at a frequency of 30 × 10⁻⁵.

^fIncludes class 1B events (defined in Figure 2), which occurred at a frequency of 11 × 10⁻⁵.