Figure 3.—

Deletion mapping of four markers flanking SKr. Markers were amplified by PCR on Chinese Spring (CS) as a reference, on the nullisomic–tetrasomic line N5B-T5D (N5B), and on the deletion lines 5BS6-0.81–1.00 (5BS6), 5BS5-0.71–0.81 (5BS5), 5BS8-0.56–0.71 (5BS8), and 5BS4-0.43–0.56 (5BS4). The two parental lines Courtot (Ct) and MP98 were analyzed for polymorphism. The sizes of the amplicons, run on agarose gel 2%, are shown (in kilobases) on the left side of the figure.