Figure 7.—

Effect of guanylic nucleotide limitation on transcription mutants. Cells were transformed with pCM189 (vector) or the tet-IMD2 plasmid, and transformants were spotted on SDcasaW medium supplemented or not with MPA (10 mg/liter) (A) or with adenine (Ade) as indicated (B). (C) Cells containing the tet-YJL070c overexpression plasmid were transformed with pCM189 (vector) or the tet-IMD2 plasmid, and transformants were spotted either on SC medium lacking uracil and leucine (C) or on SDcasaW (D).