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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Neurobiol Dis. 2008 May 2;31(2):198–208. doi: 10.1016/j.nbd.2008.04.005

Figure 4.

Figure 4

Nitration at Tyr 18 occurs in age-matched controls and in AD brains. (A) Gray matter from the frontal cortex of two AD cases (Braak stages V and VI) was homogenized and subjected to western blot analysis. Immunodetection was performed using the antibodies Tau-nY18, Tau-nY29 and 3-NT (1μg/ml) as indicated. To investigate whether nitration at Tyr 18 was an event associated with AD, soluble tau from pathologically severe AD brains (Braak stages V and VI) and normal age-matched controls (Braak stages I-III,) was biochemically enriched, resolved by SDS-PAGE, and resulting immunoblots probed with Tau-nY18 (1 μg/ml) and Tau-5 (1μg/ml) antibodies. (B) Tau-nY18 labels soluble tau isolated from both AD brains and age-matched controls. (C) Total tau within these samples was detected with the Tau-5 antibody which recognizes all forms of tau. (D) PHF-tau from severe AD brains (Braak stages V and VI) was also detected with Tau-nY18 (1μg/ml) and Tau-nY29 (1μg/ml) antibodies as indicated. In all cases 10 μg of total protein were loaded per lane.