Figure 4. Invasion of BT-549, DU145 and β1shRNA transfected cells through rBM.

rBM-coated filters were placed in Boyden chambers and media containing 5% FBS was used as a stimulus in the bottom compartment of the chambers. BT-549, DU145, BTshβ1-8 or DUshβ1-5 cells were seeded (2.5 × 10⁴ cells in 200 µl) in the top compartment of the chamber with 1% FBS and incubated at 37 °C overnight. Invasion of BT-549 and DU145 cells was also assessed in the presence of 20 µg/ml of the β1-integrin blocking antibody mAb 13. A and C. The cells that had invaded were stained, counted and imaged using 10X and 40X (Fig. inserts) objectives. B and D. Graphs are representative of at least three experiments and presented as mean ± S.D. ** P < 0.01