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. Author manuscript; available in PMC: 2009 Oct 23.
Published in final edited form as: Mol Imaging. 2008 Sep–Oct;7(5):199–213.

Figure 7. Adhesion and migration of BT-549, DU145 and β1shRNA transfected cells.

Figure 7

Adhesion assays were performed by seeding 50,000 BT-549 (A, black bars), DU145 (B, black bars), BTshβ1-8 (A, white bars) or DUshβ1-5 (B, white bars) cells in 24-well tissue culture plates coated with collagen I, collagen IV, laminin or fibronectin (5 µg/ml). After 30 min, unattached cells were removed by washing with PBS. Two mM calcein AM in PBS was added to the adherent cells for 30 min at room temperature. Fluorescent intensities were measured at 485/535 nm and expressed as relative fluorescent units (RFU). Graphs are representative of at least three experiments and data are presented as mean ± S.D. * P < 0.05 and ** P < 0.01. C. Wound healing assays were performed on 100% confluent live cells in 35 mm dishes. Prior to wounding, cells were incubated in serum-free media containing 0.1% BSA for 5–6 h. After the wounds were made, detached cells were removed by washing, and adherent cells incubated at 37°C for 24 h and then imaged by phase contrast microscopy using a Zeiss LSM 510 META NLO microscope using a 10X immersion objective. Images are representative of at least three experiments. Bar, 50 µm.