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. 2000 Aug 29;97(18):10038–10043. doi: 10.1073/pnas.97.18.10038

Figure 1.

Figure 1

CODD-PCR and initial expression analysis. (A) Statistical design of differential display PCR primers. Gene frequency of 8-mer oligonucleotides in 103 homeobox and related mRNA sequences (x axis) vs. frequency in 4,347 nonhomeobox mRNA sequences (y axis). Relative gene frequencies of 8-mer ddPCR primer cores used for amplification shown for HB-selective (arrow) and human/mouse coding region-selective (circle) primers. Color code representing number of 8-mers at x–y intersection: black, 1; blue, 2–10; green, 11–100; red, 101–1,000; purple, >1,000. (B) Autoradiogram of ddPCR products separated on 6% denaturing polyacrylamide gel. Lanes: 1, no BSN-CM; 2, with BSN-CM. Bands representing up- as well as down-regulated sequences were subcloned (arrowheads) including the 122-bp fragment used in C (arrow). (C) Northern blot analysis of in vitro 3D UB cell culture model: probe is subcloned fragment from B. Lanes: 1, serum free; 2, BSN-CM; 3, 10% serum. (D) Northern blot analysis. UB cells in 3D culture treated with CHx as in B. Lanes 1 and 2, without and with BSN-CM, respectively.