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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Neurochem. 2009 Sep 18;111(4):976–987. doi: 10.1111/j.1471-4159.2009.06385.x

Figure 6. HSP70 inhibition attenuates neuroprotection by VPA against glutamate-induced excitotoxicity in cortical neurons.

Figure 6

Cortical neurons at DIV-7 were pretreated with 25 μM KNK437 for one hour followed by treatment with 1 mM VPA or vehicle for 72 hours. They were then exposed to 100 μM glutamate in low chloride buffer for 10 minutes, followed by wash-out of glutamate and further incubation of the culture for four hours. A. HSP70 and β-actin protein levels were determined by immunoblotting. Cells were stained with MTT (B) or Hoechst dye 33258 (C) and then examined microscopically and photographed. Arrows indicate apoptotic neurons, undergoing chromatin condensation. Cell viability was quantified by MTT assay (D). Data are mean ± SEM of % of untreated control from three independent cultures. *p<0.05; ***p<0.001 between indicated groups.