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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Neurochem. 2009 Sep 18;111(4):1062–1073. doi: 10.1111/j.1471-4159.2009.06388.x

Figure 3. The role of the PB1 domain in the interaction.

Figure 3

(A) The indicated p62 constructs, tagged with either the monomeric (DsRedM) or the tetrameric (DsRedT) DsRed were co-transfected with HA-tagged A4V SOD1. The cell extracts were subjected to HA immunoprecipitation followed by Western blotting. (B). The DsRedM tagged WT, K7A/D69A or R21A/D69A p62 construct was co-transfected with HA-tagged A4V SOD1. The cell extracts were subjected to HA immunoprecipitation followed by Western blotting. (C). Demonstration of the oligomerization status of selected DsRed-p62 constructs by native gel electrophoresis followed by Western blotting. A slower band (*) and a faster band (●) in native gel electrophoresis indicate an oligomeric and a monomeric form, respectively. The panels of the DsRed blot of the native gel were obtained with 5 sec (short) and 40 sec (long) exposition times of the same membrane. The protein expression levels in extracts were assessed in all experiments. The antibody used in each experiment is specified beside each panel.