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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: J Neurochem. 2009 Sep 18;111(4):1062–1073. doi: 10.1111/j.1471-4159.2009.06388.x

Figure 6. The effect of the SMIR and LIR deletion on mutant SOD1 sequestration into p62 inclusions.

Figure 6

DsRedM-p62, DsRedM-p62-ΔSMIR, DsRedM-p62-ΔLIR or DsRedM vector and GFP-tagged A4V mutant SOD1 were co-transfected into NSC34 cells. The inclusion formation was measured by simultaneously monitoring the GFP-positive SOD1 inclusions and DsRed-positive p62 inclusions under fluorescence microscope. Cells containing DsRed, GFP, and both inclusions were counted in 10 random viewfields. The percentage of cells containing inclusions out of the transfected cells was calculated and the average values from three independent experiments are shown.