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. Author manuscript; available in PMC: 2010 Mar 17.
Published in final edited form as: Biochemistry. 2009 Mar 17;48(10):2282–2289. doi: 10.1021/bi801989c

Figure 2. M1R-mediated Ca2+ release is not affected by the Gβ5-RGS7 complex.

Figure 2

CHO-K1 cells were transfected with the M1R and Lac Z or the M1R, Gβ5 and RGS7, and the calcium responses were recorded and analyzed as described in the legend to Fig 1. (A)Representative traces from single cells. Grey trace represents cells with M1R alone, and black shows cells expressing M1R together with Gβ5-RGS7. (B) Dose-response from increasing carbachol concentration. White symbols, M1R alone, black symbols, M1R plus Gβ5-RGS7. The data represent the mean ± S.D. of three independent experiments from measurements of the amplitude of free Ca2+.