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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Signal. 2009 Sep 15;22(1):65–70. doi: 10.1016/j.cellsig.2009.09.011

Fig. 3.

Fig. 3

Resveratrol regulates the initiation phase of mRNA translation in glomerular epithelial cells. A. Cells were treated as described in Fig. 1, panel B, and immunoblotting was done with antibody against phosphorylated Ser209 on eIF4E or eIF4E antibody. Representative blots from 3 experiments are shown. Lower panel shows composite densitometric data from 3 experiments in a graph (*p<0.001 high glucose Vs control; #p<0.01 high glucose Vs high glucose+resveratrol by ANOVA). B. Glomerular epithelial cells were incubated with 30 mM glucose (Glc) and resveratol (Res) for 3 days and cell lysates (500 ug) were immunoprecipitated with eIF4G antibody and immunoblotted with eIF4E antibody. The membrane was stripped and re-probed with eIF4G antibody. A representative blots from 3 experiments are shown. Lower panel shows composite densitometric data from 3 experiments in a graph (*p<0.01 high glucose Vs control; #p<0.01 high glucose Vs high glucose+resveratrol by ANOVA).