Table 2.

Characteristics of the BMSCs derived from MM patients and control subjects

Parameter	MM patients n=16	Controls n=11
MoAb CD38+/CD138+ (% of positive cells)	0	0
MoAb *CD166+ (% of positive cells)	75.60±11.95	86.6±8.5
MoAb CD14+ (% of positive cells)	13.1±6.0	9.8±9.1
TRAP (µmol of pNP/1×105 cells)	33.7±3.0	44.9±16.4
ALP (µmol of pNP/1×105 cells)	45.78±12.20	38.47±14.35

*Marker for human BM stroma fibroblast [42].

Cells were analyzed by three-color flow cytometry using MoAb anti-CD38 PE-Cy5 and CD138 FITC, CD14 Tri-Color, and CD166 FITC using a Becton Dickinson FACS-Calibur.

Tartrate-resistant acid phosphatase (TRAP) was measured by microplate colorimetric assay according to the method described by Nakasato et al. [31].

Alkaline phosphatase (ALP) activity was measured by colorimetric assay as described by Coelho and Fernandez [6].