Fig. 5.

Effect of MNA and NA on the production of PGE2 and COX-2 expression by LPS-stimulated macrophages. Cells stimulated with LPS (100 ng/ml) were exposed to MNA and NA in the culture medium for 24 h and then both the medium and the cells were collected for further assays. A — the release of PGE₂; results are expressed as the percentage of the PGE₂ production by control Mϕ ±SEM. Data were calculated from two separate experiments. B — the expression of COX-2; the data are normalized to constitutively expressed β-actin protein ±SEM. Densitometric analysis of bands from two independent experiments. *p<0.05.