Table. 1.
Distribution of MNA and NA added to the culture of LPS-stimulated macrophages
Exogenous compounds (µM)a | Final concentrationb | The ratio of medium to cytosol concentration | |
---|---|---|---|
medium | cytosol | ||
MNA (0.0) | <0.1 | <0.5 | ND |
MNA (10.000) | 7991±461 | 223±32 | 35.8 |
NA (0.0) | 9±1 | 47±3 | ND |
NA (4.000) | 3598±87 | 420±26 | 8.57 |
aMNA or NA was added to the culture of LPS-stimulated Mϕ at the concentrations indicated in brackets. bAfter 24 h, supernatant and Mϕ were collected separately and the concentrations of extracellular (medium) and intracellular (cytosol) MNA and NA were measured as described in Materials and Methods. ND — not done.