Table 1.
shRNA | Sequences of oilgonucleotides | Target site on pokemon mRNA (GenBank accession number NM_01 5898) |
---|---|---|
1 | F1: 5′-ccgtgtacgagatcgacttcttcaagagagaagtcgatctcgtacacgtttttt-3′ | 383–401 nt |
R1: 3′-ccggggcacatgctctagctgaagaagttctctcttcagctagagcatgtgcaaaaaatcga-5′ | ||
2 | F2: 5′-ccggcttagacttctatgggttcaagagacccatagaagtctaagccgtttttt-3′ | 809–827 nt |
R2: 3′-ccggggccgaatctgaagatacccaagttctctgggtatcttcagattcggcaaaaaatcga-5′ | ||
3 | F3: 5′-cgggcgtcatggactactacttcaagagagtagtagtccatgacgccctttttt-3′ | 1202–1200 nt |
R3: 3′-ccgggcccgcagtacctgatgatgaagttctctcatcatcaggtactgcgggaaaaaatcga-5′ |
shRNA encoding cassettes 1–3 were formed by annealing two corresponding oligonucleotides, respectively. F stands for forward and R for reverse.