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Rb and phospho-Rb expression is reduced in HTR-8 cells infected with P. gingivalis. HTR-8 cells were infected with P. gingivalis at the times and moi indicated. Lysates of infected and non-infected cells were analysed by immunoblotting with antibodies to phosphorylated (phospho-) Rb or total Rb. Blots were analysed by scanning densitometry and ratios of phospho-Rb/Rb and of Rb/β-actin relative to the zero time point determined. Data are representative of three independent experiments.
