Figure 3.
Endothelial density determined by CD31 staining (brown cytoplasmic staining) 7 days after myocardial infarction in the injury border region of the (A) C57BL/6 and (B) MRL/MpJ mouse strain (Bar = 50 μm). The insets show vessel structure (Inset Bar = 10 μm). Example endothelial cells are indicated by (+) and CD31-negative cells are marked with (−). CD31 staining in the infarcted region of C57BL/6 and MRL/MpJ mouse strains indicates similar vessel density between the strains. No significant differences exist in endothelial cell density between the two strains in (C) the uninjured myocardium and (F) the infarct scar to 90 days. Vessel density measurements (G) are also equivalent between the two strains.
