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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Cardiovasc Pathol. 2007 Mar 21;17(1):6–13. doi: 10.1016/j.carpath.2007.01.005

Figure 4.

Figure 4

Cardiac scar morphology by hematoxylin and eosin and picrosirius red/fast green staining in response to a 10 second direct cryoinjury is equivalent in the MRL/MpJ and C57BL/6 mouse 28 days (A) and 7 days (B) after injury. (Bar = 1mm) Extent of cryoinjury includes the right ventricle in each intervention, indicating lack of regeneration in that area. (C) Scar volume measured from serial sirius red stained sections is equivalent between each strain at both 1 and 4 weeks. (D) Each strain showed both sharp and jagged transitions at the infarct border zones owing to the muscle fiber orientation at that area of the tissue section. (Bar = 50 μm.) (E) No proliferating cardiomyocytes were found 7 days after cryoinjury as measured by immunostaining for BrdU (brown nuclei) and sarcomeric actin (red cytoplasm). (Bars = 50 and 20 μm.)