Table 1.

Thermodynamic Parameters of Duplex Formation for R5a Labeled CS Duplexes

labeled position	ΔH°b (kcal/mol)	ΔS°b (cal/(mol·K))	ΔG°37°cc (kcal/mol)	ΔΔG°37°cd (kcal/mol)
(A) Wild-Type CS Duplexa
none	−69 ± 2	−192 ± 8	−9.4
CS7	−68 ± 5	−191 ± 17	−9.2	0.2
CS9	−63 ± 5	−174 ± 15	−8.8	0.6
CS12	−67 ± 2	−186 ± 7	−9.2	0.2
CS14	−64 ± 2	−176 ± 5	−9.1	0.3
(B) Mutant CS Duplexe
none	−75 ± 3	−214 ± 8	−9.1
CS2_1A	−80 ± 1	−227 ± 5	−9.4	−0.3
CS24_12T	−78 ± 3	−222 ± 9	−9.0	0.1

^aDNA sequence as shown in Figure 1A.

^bAverages and errors were obtained based on results from multiple sets of melting measurements.

^cΔG°_37°C = ΔH° − 310 K × ΔS°. Averages of ΔG°_37°C value from multiple melting measurements are reported, with errors estimated to be less than ± 0.1 kcal/mol.

^dΔΔG°_37°C = ΔG°_37°C (labeled) − ΔG°_37°C (unlabeled).

^eDNA sequence modified at the duplex terminus: C1/G24 to A1/T24.