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. 2009 Jul 21;587(Pt 18):4425–4439. doi: 10.1113/jphysiol.2009.175075

Figure 2. Sample recordings from taste cells in which expression of gustducin was detected by single cell RT-PCR.

Figure 2

Upper panels show taste responses to five basic taste stimuli. Dotted lines show the onset of taste stimulation. Lower panels show the gel electrophoretic detection of expression of SNAP25, gustducin, T1r3 and β-actin by multiplex single cell RT-PCR. Purified mRNA from a single taste bud and electrode solution without sample were used as positive control (PC) and negative control (NC), respectively. The predicted sizes of PCR products were the following: SNAP25 (304 bp), gustducin (341 bp), T1r3 (343 bp), β-actin (370 bp).